ДИНАМІКА ФУНКЦІОНАЛЬНИХ ПОРУШЕНЬ ПРИ РІЗНИХ ВАРІАНТАХ ТКАНИННОЇ ТРАНСПЛАНТАЦІЇ З МЕТОЮ АКТИВАЦІЇ АНГІОГЕНЕЗУ НА МОДЕЛІ ФОКАЛЬНОЇ ЦЕРЕБРАЛЬНОЇ ІШЕМІЇ В ЩУРІВ

Анотація

Summary. We have analyzed the dynamics of neurological impairment in rats with focal cerebral ischemia under different variants of tissue transplantation aimed at activation of angiogenesis in the zone of brain ischemic lesion. 72 white male rats were used in the research, divided into 4 groups: “control” (n=18, permanent right middle cerebral artery occlusion), “experiment” (n=18 each): “experiment” №1 - intracerebral allogenic embryonic neural tissue grafting, 2 days after MCAO (n=18), №2 - intracerebral allogenic bone marrow grafting, 2 days after MCAO (n=18), №3 - intracerebral phosphate buffer infusion, on the day after occlusion (n=18). Assessment of global neurologic deficit, motor, sensory and reflectory impairment was performed using our protocol for behavioral testing on 1, 3, 7, 14, 21 and 28 day after inducing cerebral ischemia. In groups “experiment” №1 and “experiment” №2 substantial regression of neurologic deficit has been observed from the 3rd (motor asymmetry index: “control” - 73,4±1,6, “experiment” №1 - 66,6±1,4, №2 - 59±2, p<0,001) to the 28th day after occlusion (“control” - 53,9±1,3, “experiment” №1 - 36,8±0,8, №2 - 21,9±1, p<0,001). However no significant differences were observed between groups “control” and “experiment” №3 (p=0,083 according to Bonferroni and p=0,105 according to Scheffe post-hoc tests). Furthermore, bone marrow grafting was proved to be more effective than embryonic neural tissue that could be associated with its influence on postischemic cerebral angio- and synaptogenesis. Thus, functional improvement in rats after focal cerebral ischemia may indicate the efficiency of tissue transplantation under experimental conditions and could be an important prerequisite for further clinical trials of bone marrow and embryonic neural tissue grafting in patients with ischemic stroke.